Roundworms live in the intestines of dogs and cats. Hookworm polymerase chain reaction species differentiation. Toxoplasmosis is a disease caused by the toxoplasma, a zoonotic parasite that lives in the intestines of. Humans are infected because the hookworm larva matures into a stage that can penetrate the skin. This occurs most often by walking barefoot in contaminated areas or by accidental ingestions of soil or feces.
#Zoonotic hookworms in humans skin
On the other hand, PCR is known to be a sensitive, specific, and a reliable investigative tool which can help in diagnosis as well as in species differentiation. Hookworms on occasion cause creeping lesions in the superficial layers of the human skin that have been designated as cutaneous larva migrans for the purpose of contrasting the condition with visceral larva migrans. Humans become infected by coming into contact with contaminated soil or feces. Coproculture is time-consuming and needs the expertise to differentiate the species. Dogs and cats play a significant role as reservoir. Stool microscopy is the major mode of detection, but it has a higher false negative rate. strercoralis was helminth infection in cats which has potential zoonotic disease to human.
True positivity by agar plate culture was 83.3% and false positivity rate was 16.6%. Sensitivity of Kato-Katz method was 87.5% and specificity was 100%. Sensitivity of stool microscopy was found to be 81.2% and the specificity was 100%. Majority of the infections were mild intensity infections. Highest hookworm prevalence was seen in 20-30 years of age group. The prevalence of hookworm was estimated as 7.6%. This study was performed to evaluate the utility of polymerase chain reaction (PCR) for detection of hookworm infections.Ī total of 209 stool samples were collected and subjected to stool microscopy, Kato-Katz method to identify the intensity of the infection, coproculture for 元 larval identification and species differentiation and semi-nested PCR with sequencing. Hence, species differentiation is imperative because the eradication of both anthroponotic and zoonotic hookworm depends on the concurrent human and animal health programs, hygienic practices, and mass drug administration for humans and dogs. Among the zoonotic hookworm species, only Ancylostoma ceylanicum causes potent human infections where dogs and cats act as reservoir of infection. Human hookworm infection is caused mainly by Necator americanus and Ancylostoma duodenale.
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